The successful implementation of photodynamic therapy (PDT)‐based regimens depends on an improved understanding of the dosimetric and biological factors that govern therapeutic variability. Here, the kinetics of tumor destruction and regrowth are characterized by systematically varying benzoporphyrin derivative (BPD)‐light combinations to achieve fixed PDT doses (M × J cm −2).

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Three endpoints were used to evaluate treatment response: (1) Viability evaluated every 24 h for 5 days post‐PDT; (2) Photobleaching assessed immediately post‐PDT; and (3) Caspase‐3 activation determined 24 h post‐PDT. The specific BPD‐light parameters used to construct a given PDT dose significantly impact not only acute cytotoxic efficacy, but also treatment durability.

For each dose, PDT with 0.25 μM BPD produces the most significant and sustained reduction in normalized viability compared to 1 and 10 μM BPD. Percent photobleaching correlates with normalized viability for a range of PDT doses achieved within BPD concentrations. To produce a cytotoxic response with 10 μM BPD that is comparable to 0.25 and 1 μM BPD a reduction in irradiance from 150 to 0.5 mW cm −2 is required. Activated caspase‐3 does not correlate with normalized viability. The parameter‐dependent durability of outcomes within fixed PDT doses provides opportunities for treatment customization and improved therapeutic planning. SPIE BiOS San Francisco, California, United States Saturday 7 February 2015 SPIE Proceedings 0277786X Optical Elastography and Tissue Biomechanics II 9327 SPIE, (2015).

Jones, William Hanna and Jonathan P. Celli Mapping dynamic mechanical remodeling in 3D tumor models via particle tracking microrheology, (2015). 93270L, 10.11282 • Daria S. Kuznetsova, Marina V. Shirmanova, Varvara V.

Dudenkova, Pavel V. Subochev, Ilya V.

Turchin, Elena V. Zagaynova, Sergey A. Lukyanov, Boris E. Prezentaciya portfolio uchitelya nachaljnih klassov v kazahstane.

Shakhov and Vladislav A. Kamensky, Photobleaching and phototoxicity of KillerRed in tumor spheroids induced by continuous wave and pulsed laser illumination, Journal of Biophotonics, 8, 11-12, (952-960), (2015). Filename Description image/eps, 1.2 MB Figure S1.

At all PDT doses, 0.25 μM BPD exhibits significantly more photobleaching than 1 μM and 10 μM BPD. At all PDT doses, 0.25 μM BPD photobleached significantly more than 1 and 10 μM BPD ( P. Vizitka na anglijskom yazike obrazec 1.

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